Oligomycin A (MCH 32) is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.

F0F1-ATPase:1 μM(Ki)

方法：人黑色素瘤细胞 WM3734 用 Oligomycin A (0.01-1 µg/mL) 处理 24 h，取培养上清液中测量乳酸的产生。
结果：Oligomycin A 引起 WM3734 细胞中乳酸分泌的增加。[1]
方法：人肺癌细胞 H1299 用 Oligomycin A (0.1-1000 ng/mL) 处理 100 min，使用 Oxygen Biosensor System plates 检测细胞呼吸。
结果：在 100 和 1000 ng/mL 时，Oligomycin A 在大约一小时内完全抑制细胞呼吸。[2]

方法：为检测抗炎活性，将 Oligomycin A (0.25 mg/kg) 腹腔注射给银屑病模型的 C57BL6 小鼠，每天一次，持续五天。
结果：Oligomycin A 减少了耳朵厚度、角质形成细胞增殖和免疫细胞浸润。[3]

ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.(Only for Reference)